Collection and storage of pollen from Salix (Salicaceae).
Identifieur interne : 001F83 ( Main/Exploration ); précédent : 001F82; suivant : 001F84Collection and storage of pollen from Salix (Salicaceae).
Auteurs : Richard F. Kopp [États-Unis] ; Charles A. Maynard ; Patricia Rocha De Niella ; Lawrence B. Smart ; Lawrence P. AbrahamsonSource :
- American journal of botany [ 0002-9122 ] ; 2002.
Abstract
Genetic improvement of willows through traditional breeding can be facilitated by pollen collection and storage so that female flower receptivity need not be synchronized with pollen shed for breeding. Two experiments were completed to test the effectiveness of various organic solvents for willow pollen collection. In the first experiment, seven pollen collection treatments and an untreated control were tested with two willow clones. The other experiment tested three treatments that showed promise in the initial experiment and an untreated control with eight willow clones. Toluene and carbon tetrachloride were effective for pollen extraction, with average pollen germination percentages that were >15%, but both chemicals reduced pollen viability by 10-20% compared with an untreated control based on in vitro germination tests. Pollen extracted with carbon tetrachloride or toluene was successfully used in controlled pollination, and >100 new families were produced with this technique. Pollen viability remained high after 18 mo of storage at -20°C. Based on our results, toluene is the preferred solvent for future willow pollen extractions because it is as effective as carbon tetrachloride, is not a known carcinogen, and is less expensive.
DOI: 10.3732/ajb.89.2.248
PubMed: 21669733
Affiliations:
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Kopp</name><affiliation wicri:level="2"><nlm:affiliation>State University of New York College of Environmental Science and Forestry, Faculty of Environmental and Forest Biology, Syracuse, New York 13210 USA;</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>State University of New York College of Environmental Science and Forestry, Faculty of Environmental and Forest Biology, Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Maynard, Charles A" sort="Maynard, Charles A" uniqKey="Maynard C" first="Charles A" last="Maynard">Charles A. Maynard</name></author><author><name sortKey="Rocha De Niella, Patricia" sort="Rocha De Niella, Patricia" uniqKey="Rocha De Niella P" first="Patricia" last="Rocha De Niella">Patricia Rocha De Niella</name></author><author><name sortKey="Smart, Lawrence B" sort="Smart, Lawrence B" uniqKey="Smart L" first="Lawrence B" last="Smart">Lawrence B. Smart</name></author><author><name sortKey="Abrahamson, Lawrence P" sort="Abrahamson, Lawrence P" uniqKey="Abrahamson L" first="Lawrence P" last="Abrahamson">Lawrence P. Abrahamson</name></author></analytic><series><title level="j">American journal of botany</title><idno type="ISSN">0002-9122</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genetic improvement of willows through traditional breeding can be facilitated by pollen collection and storage so that female flower receptivity need not be synchronized with pollen shed for breeding. Two experiments were completed to test the effectiveness of various organic solvents for willow pollen collection. In the first experiment, seven pollen collection treatments and an untreated control were tested with two willow clones. The other experiment tested three treatments that showed promise in the initial experiment and an untreated control with eight willow clones. Toluene and carbon tetrachloride were effective for pollen extraction, with average pollen germination percentages that were >15%, but both chemicals reduced pollen viability by 10-20% compared with an untreated control based on in vitro germination tests. Pollen extracted with carbon tetrachloride or toluene was successfully used in controlled pollination, and >100 new families were produced with this technique. Pollen viability remained high after 18 mo of storage at -20°C. Based on our results, toluene is the preferred solvent for future willow pollen extractions because it is as effective as carbon tetrachloride, is not a known carcinogen, and is less expensive.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">21669733</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2011</Year><Month>06</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0002-9122</ISSN><JournalIssue CitedMedium="Print"><Volume>89</Volume><Issue>2</Issue><PubDate><Year>2002</Year><Month>Feb</Month></PubDate></JournalIssue><Title>American journal of botany</Title><ISOAbbreviation>Am J Bot</ISOAbbreviation></Journal><ArticleTitle>Collection and storage of pollen from Salix (Salicaceae).</ArticleTitle><Pagination><MedlinePgn>248-52</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3732/ajb.89.2.248</ELocationID><Abstract><AbstractText>Genetic improvement of willows through traditional breeding can be facilitated by pollen collection and storage so that female flower receptivity need not be synchronized with pollen shed for breeding. Two experiments were completed to test the effectiveness of various organic solvents for willow pollen collection. In the first experiment, seven pollen collection treatments and an untreated control were tested with two willow clones. The other experiment tested three treatments that showed promise in the initial experiment and an untreated control with eight willow clones. Toluene and carbon tetrachloride were effective for pollen extraction, with average pollen germination percentages that were >15%, but both chemicals reduced pollen viability by 10-20% compared with an untreated control based on in vitro germination tests. Pollen extracted with carbon tetrachloride or toluene was successfully used in controlled pollination, and >100 new families were produced with this technique. Pollen viability remained high after 18 mo of storage at -20°C. 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Abrahamson</name><name sortKey="Maynard, Charles A" sort="Maynard, Charles A" uniqKey="Maynard C" first="Charles A" last="Maynard">Charles A. Maynard</name><name sortKey="Rocha De Niella, Patricia" sort="Rocha De Niella, Patricia" uniqKey="Rocha De Niella P" first="Patricia" last="Rocha De Niella">Patricia Rocha De Niella</name><name sortKey="Smart, Lawrence B" sort="Smart, Lawrence B" uniqKey="Smart L" first="Lawrence B" last="Smart">Lawrence B. Smart</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Kopp, Richard F" sort="Kopp, Richard F" uniqKey="Kopp R" first="Richard F" last="Kopp">Richard F. Kopp</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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